Hotspot

ndhF-rpl32

SSC · 111496-112307 · 812 bp

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Pi 0.108

MarkerSeek score 79.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 812
variable_sites: 70
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1501-1611 bp	1.0	89.1
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	CACCATAGCGGCTTATTCGA	50.0	50.0	58.1	57.9	1474-1584 bp	1.0	89.0
ndhF-rpl32_p3	GCCCACATACGACGAAGATT	TCACCATAGCGGCTTATTCG	50.0	50.0	58.1	57.9	1475-1585 bp	1.0	89.0
ndhF-rpl32_p4	GCCCACATACGACGAAGATT	TCGAACCGAGATGCTTTAGC	50.0	50.0	58.1	58.1	1637 bp	0.5	58.9
ndhF-rpl32_p5	AGGAAAAGCCCACATACGAC	TCGAACCGAGATGCTTTAGC	50.0	50.0	57.9	58.1	1644 bp	0.5	58.5