Hotspot

rps8-rpl14

LSC · 80732-81250 · 519 bp

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Pi 0.128

MarkerSeek score 83.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 63
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCCTCTCGATCCGTGATTA	CCCTAGCACCCGAGGTATTA	50.0	55.0	58.0	58.0	905-919 bp	1.0	89.6
rps8-rpl14_p2	AGCCTCTCGATCCGTGATTA	CCGAGGTGGACAAATATGGG	50.0	55.0	58.0	58.0	1607-1619 bp	1.0	86.8
rps8-rpl14_p3	AAACTTCTCCGCCGATTCTC	CCCTAGCACCCGAGGTATTA	50.0	55.0	58.0	58.0	933 bp	0.5	59.9
rps8-rpl14_p4	AAACTTCTCCGCCGATTCTC	CCGAGGTGGACAAATATGGG	50.0	55.0	58.0	58.0	1635 bp	0.5	59.6
rps8-rpl14_p5	AAACTTCTCCGCCGATTCTC	CCTACCGAAACACGTATGGG	50.0	55.0	58.0	58.1	1580 bp	0.5	59.4