Hotspot

cemA-psaI

LSC · 67955-69330 · 1376 bp

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Pi 0.128
MarkerSeek score 84.70
Primer available yes
Label cemA-psaI

Feature Metadata

label_name
cemA-psaI
feature_type
igs
region
LSC
length_bp
1376
variable_sites
160
pi
0.128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-psaI_p1 AAGGGGAATGAATGTTTTCT TGCAATGGCTTCTTTATTTCTTCA 35.0 33.3 52.6 57.7 1405-1455 bp 1.0 52.0
cemA-psaI_p2 AGGGGAATGAATGTTTTCTT TGCAATGGCTTCTTTATTTCTTCA 35.0 33.3 52.6 57.7 1404-1454 bp 1.0 52.0
cemA-psaI_p3 AAGGGGAATGAATGTTTTCTT TGCAATGGCTTCTTTATTTCTTCA 33.3 33.3 53.5 57.7 1405-1455 bp 1.0 52.0
cemA-psaI_p4 AGGGGAATGAATGTTTTCTTT TGCAATGGCTTCTTTATTTCTTCA 33.3 33.3 53.5 57.7 1404-1454 bp 1.0 52.0
cemA-psaI_p5 AGGGGAATGAATGTTTTCTTTT TGCAATGGCTTCTTTATTTCTTCA 31.8 33.3 54.4 57.7 1404-1454 bp 1.0 52.0