Hotspot

psbJ-petA

LSC · 65098-66092 · 995 bp

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Pi 0.0832

MarkerSeek score 82.20

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 79
pi: 0.0832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	GGATTGGGCTCATCCTTGTA	TGAGAAGGTTCAATTGTCCGA	50.0	42.9	57.0	57.2	1035-1050 bp	1.0	67.3
psbJ-petA_p2	GGATTGGGCTCATCCTTGTAG	TGAGAAGGTTCAATTGTCCGA	52.4	42.9	58.1	57.2	1035-1050 bp	1.0	66.3
psbJ-petA_p3	GGATTGGGCTCATCCTTGTA	TGAGAAGGTTCAATTGTCCGAA	50.0	40.9	57.0	57.9	1035-1050 bp	1.0	64.7
psbJ-petA_p4	GGATTGGGCTCATCCTTGTA	TTGAGAAGGTTCAATTGTCCGA	50.0	40.9	57.0	57.9	1036-1051 bp	1.0	64.7
psbJ-petA_p5	GGATTGGGCTCATCCTTGTAG	TGAGAAGGTTCAATTGTCCGAA	52.4	40.9	58.1	57.9	1035-1050 bp	1.0	63.8