Hotspot

trnQ-UUG-accD

LSC · 56407-57697 · 1291 bp

Back to Clitoria

Pi 0.0892

MarkerSeek score 82.00

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: LSC
length_bp: 1291
variable_sites: 108
pi: 0.0892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	CAATGTTCCTGACACTCGCT	55.0	50.0	58.1	58.2	1528-1535 bp	1.0	88.1
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	CCCAATGTTCCTGACACTCG	55.0	55.0	58.1	58.6	1530-1537 bp	1.0	85.2
trnQ-UUG-accD_p3	CCCCCAAAAACCCTCTTTGA	CAATGTTCCTGACACTCGCT	50.0	50.0	57.9	58.2	1833 bp	0.5	57.6
trnQ-UUG-accD_p4	CAAAGTGAAAATGGGGCGTG	CAATGTTCCTGACACTCGCT	50.0	50.0	58.2	58.2	1594 bp	0.5	56.7
trnQ-UUG-accD_p5	TCAAAGTGAAAATGGGGCGT	CAATGTTCCTGACACTCGCT	45.0	50.0	58.3	58.2	1595 bp	0.5	56.0