Hotspot

atpA-trnR-UCU

LSC · 52354-52721 · 368 bp

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Pi 0.126
MarkerSeek score 67.60
Primer available yes
Label atpA-trnR-UCU

Feature Metadata

label_name
atpA-trnR-UCU
feature_type
igs
region
LSC
length_bp
368
variable_sites
29
pi
0.126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.62
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-trnR-UCU_p1 GCATTCGCGTCTTTTGGAAA AGCCTTCCAAGCTAACGATG 45.0 50.0 58.0 58.0 1271-1472 bp 1.0 87.8
atpA-trnR-UCU_p2 GCATTCGCGTCTTTTGGAAA ACCCTTAGCCTTCCAAGCTA 45.0 50.0 58.0 58.0 1277-1478 bp 1.0 87.7
atpA-trnR-UCU_p3 GGTCGTGAAGCTTATCCAGG AGCCTTCCAAGCTAACGATG 55.0 50.0 58.1 58.0 1306-1507 bp 1.0 87.6
atpA-trnR-UCU_p4 GTCGTGAAGCTTATCCAGGG AGCCTTCCAAGCTAACGATG 55.0 50.0 58.1 58.0 1305-1506 bp 1.0 87.6
atpA-trnR-UCU_p5 CCAGGTCGTGAAGCTTATCC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.1 58.0 1309-1510 bp 1.0 87.5