Hotspot

petN-trnC-GCA

LSC · 34108-34851 · 744 bp

Back to Clitoria

Pi 0.132

MarkerSeek score 82.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 95
pi: 0.132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TACCATTAAAGCAGCCCAGG	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.6	58.1	812-896 bp	1.0	86.1
petN-trnC-GCA_p2	TACCATTAAAGCAGCCCAGG	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.6	58.4	830-914 bp	1.0	83.9
petN-trnC-GCA_p3	GGGTTACGTGGTTCCTCATT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.5	58.1	1234 bp	0.5	55.5
petN-trnC-GCA_p4	TACCATTAAAGCAGCCCAGG	TTCCCCCATTTCCATCCTCA	50.0	50.0	57.6	58.3	1427 bp	0.5	54.1
petN-trnC-GCA_p5	TACCATTAAAGCAGCCCAGG	CCCCATTTCCATCCTCACTC	50.0	55.0	57.6	57.6	1424 bp	0.5	53.7