Hotspot

psbM-petN

LSC · 32848-34017 · 1170 bp

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Pi 0.0899

MarkerSeek score 79.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1170
variable_sites: 91
pi: 0.0899
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TTCAATTGGTCAGAGCACCG	CCTGGGCTGCTTTAATGGTA	50.0	50.0	58.5	57.6	1680-1843 bp	1.0	82.1
psbM-petN_p2	TTCAATTGGTCAGAGCACCG	TCCGTTTACTGACTGGGTCT	50.0	50.0	58.5	58.0	2361 bp	0.5	56.1
psbM-petN_p3	TTCAATTGGTCAGAGCACCG	CTGGGTCTTGATAGGTCGGA	50.0	55.0	58.5	58.2	2349 bp	0.5	54.4
psbM-petN_p4	TTCAATTGGTCAGAGCACCG	TGGGTCTTGATAGGTCGGAT	50.0	50.0	58.5	57.5	2348 bp	0.5	52.4
psbM-petN_p5	TTCAATTGGTCAGAGCACCG	ACTGGGTCTTGATAGGTCGG	50.0	55.0	58.5	58.5	2350 bp	0.5	52.1