Hotspot

trnY-GUA-trnD-GUC

LSC · 31755-32235 · 481 bp

Back to Clitoria

Pi 0.124

MarkerSeek score 81.20

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 49
pi: 0.124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	467-551 bp	1.0	86.0
trnY-GUA-trnD-GUC_p2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	466-550 bp	1.0	86.0
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	488-572 bp	1.0	84.8
trnY-GUA-trnD-GUC_p4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	487-571 bp	1.0	84.6
trnY-GUA-trnD-GUC_p5	CGGACTGTAAATTCGTTGGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.5	58.5	483-567 bp	1.0	83.5