Hotspot

trnT-GGU-trnE-UUC

LSC · 30726-31531 · 806 bp

Back to Clitoria

Pi 0.0877

MarkerSeek score 81.70

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 67
pi: 0.0877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	TTTCATGGTGGATCGGCAAA	50.0	45.0	58.0	58.1	1075-1108 bp	1.0	88.3
trnT-GGU-trnE-UUC_p2	CCATGGCGTTACTCTACCAC	TTTCATGGTGGATCGGCAAA	55.0	45.0	58.1	58.1	1054-1087 bp	1.0	88.0
trnT-GGU-trnE-UUC_p3	GAACCGATGACTTACGCCTT	CCCAGGAACAGGAAGAATCG	50.0	55.0	58.0	58.0	1003 bp	0.5	59.7
trnT-GGU-trnE-UUC_p4	CCATGGCGTTACTCTACCAC	CCCAGGAACAGGAAGAATCG	55.0	55.0	58.1	58.0	982 bp	0.5	59.2
trnT-GGU-trnE-UUC_p5	TGGCGTTACTCTACCACTGA	CCCAGGAACAGGAAGAATCG	50.0	55.0	58.1	58.0	979 bp	0.5	59.1