Hotspot

psbD-trnT-GGU

LSC · 29184-30653 · 1470 bp

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Pi 0.0729

MarkerSeek score 81.80

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1470
variable_sites: 100
pi: 0.0729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AATTTACCAAGAGCTATAGTCA	GGCGTAAGTCATCGGTTCAA	31.8	50.0	52.3	58.3	1506-1528 bp	1.0	49.3
psbD-trnT-GGU_p2	AATTTACCAAGAGCTATAGTCA	GTGGTAGAGTAACGCCATGG	31.8	55.0	52.3	58.1	1529-1551 bp	1.0	49.2
psbD-trnT-GGU_p3	AATTTACCAAGAGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	31.8	50.0	52.3	58.1	1532-1554 bp	1.0	49.2
psbD-trnT-GGU_p4	AAATTTACCAAGAGCTATAGTCA	GTGGTAGAGTAACGCCATGG	30.4	55.0	53.2	58.1	1530-1552 bp	1.0	49.2
psbD-trnT-GGU_p5	AAATTTACCAAGAGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	30.4	50.0	53.2	58.1	1533-1555 bp	1.0	49.2