Hotspot

ycf3-psaA

LSC · 19232-20192 · 961 bp

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Pi 0.0894
MarkerSeek score 79.80
Primer available yes
Label ycf3-psaA

Feature Metadata

label_name
ycf3-psaA
feature_type
igs
region
LSC
length_bp
961
variable_sites
74
pi
0.0894
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-psaA_p1 TCACAGGGCGTTTTGAATAA ACTTCTGGTTCCGGTGAA 40.0 50.0 55.6 55.6 918-1010 bp 1.0 50.1
ycf3-psaA_p2 ATCACAGGGCGTTTTGAATA ACTTCTGGTTCCGGTGAA 40.0 50.0 55.3 55.6 919-1011 bp 1.0 50.1
ycf3-psaA_p3 ATCACAGGGCGTTTTGAATAA ACTTCTGGTTCCGGTGAA 38.1 50.0 56.1 55.6 919-1011 bp 1.0 50.1
ycf3-psaA_p4 ATCACAGGGCGTTTTGAAT ACTTCTGGTTCCGGTGAA 42.1 50.0 55.4 55.6 919-1011 bp 1.0 50.1
ycf3-psaA_p5 TCACAGGGCGTTTTGAATA ACTTCTGGTTCCGGTGAA 42.1 50.0 54.7 55.6 918-1010 bp 1.0 50.1