Hotspot

ndhJ-trnF-GAA

LSC · 13111-13760 · 650 bp

Back to Clitoria

Pi 0.0883

MarkerSeek score 81.70

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 56
pi: 0.0883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	ACTTTTATGAAATACAAGATGCT	CCTCGTGTCACCAGTTCAAA	26.1	50.0	52.4	58.1	713-716 bp	1.0	48.1
ndhJ-trnF-GAA_p2	ACTTTTATGAAATACAAGATGCT	CTGGTAGAGCAGAGGACTGA	26.1	55.0	52.4	57.9	737-740 bp	1.0	47.8
ndhJ-trnF-GAA_p3	ACTTTTATGAAATACAAGATGCT	GGGATAGCTCAGCTGGTAGA	26.1	55.0	52.4	58.0	749-752 bp	1.0	47.7
ndhJ-trnF-GAA_p4	ACTTTTATGAAATACAAGATGCT	AGAATGGTCGGGATAGCTCA	26.1	50.0	52.4	57.9	758-761 bp	1.0	47.6
ndhJ-trnF-GAA_p5	ACTTTTATGAAATACAAGATGCT	GCTTCAAGAATGGTCGGGAT	26.1	50.0	52.4	58.0	764-767 bp	1.0	47.5