Hotspot

ndhF

SSC · 108373-110604 · 2232 bp

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Pi 0.016
MarkerSeek score 56.90
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2232
variable_sites
104
pi
0.016
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GAGAATCAGGACAATTCAAGA TGACTCCTTTCAAAAGAAGT 38.1 35.0 53.3 52.2 2321-2327 bp 1.0 43.8
ndhF_p2 GAGAATCAGGACAATTCAAGA TCCTTTCAAAAGAAGTAAAATCCGA 38.1 32.0 53.3 57.2 2317-2323 bp 1.0 43.8
ndhF_p3 GAGAATCAGGACAATTCAAGA TTGACTCCTTTCAAAAGAAGT 38.1 33.3 53.3 53.2 2322-2328 bp 1.0 43.8
ndhF_p4 GAGAATCAGGACAATTCAAGA CTCCTTTCAAAAGAAGTAAAATCCG 38.1 36.0 53.3 56.9 2318-2324 bp 1.0 43.8
ndhF_p5 GAGAATCAGGACAATTCAAGA CCTTTCAAAAGAAGTAAAATCCGA 38.1 33.3 53.3 55.9 2316-2322 bp 1.0 43.8