Hotspot

ndhD

SSC · 113349-114878 · 1530 bp

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Pi 0.00766

MarkerSeek score 51.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 39
pi: 0.00766
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GAATTAGTGAGAGCCCCGTG	TGATACCTTGGGATGGGTGT	55.0	50.0	58.1	58.0	1946 bp	0.125	44.4
ndhD_p2	GTGAATTAGTGAGAGCCCCG	TGATACCTTGGGATGGGTGT	55.0	50.0	58.1	58.0	1948 bp	0.125	44.4
ndhD_p3	TTGGAGTTGGGACCCAAAAG	TGATACCTTGGGATGGGTGT	50.0	50.0	57.9	58.0	2230 bp	0.125	44.2
ndhD_p4	CTAACGAAGCATGGGGATCC	TGATACCTTGGGATGGGTGT	55.0	50.0	58.1	58.0	2252 bp	0.125	43.9
ndhD_p5	GAATTAGTGAGAGCCCCGTG	ATGTACCCAATGTGTCCGTG	55.0	50.0	58.1	57.9	1990 bp	0.125	43.7