Hotspot

rps15-ycf1

SSC · 121133-121543 · 411 bp

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Pi 0.0284

MarkerSeek score 73.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 411
variable_sites: 38
pi: 0.0284
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACCCTCTATTTTCTTCGTTTTCT	TCCGCGATTGAAATTTTGTGA	34.8	38.1	55.9	57.1	492-536 bp	1.0	49.2
rps15-ycf1_p2	GACCCTCTATTTTCTTCGTTTTCT	TCCGCGATTGAAATTTTGTGA	37.5	38.1	57.0	57.1	493-537 bp	1.0	49.1
rps15-ycf1_p3	GACCCTCTATTTTCTTCGTT	TCCGCGATTGAAATTTTGTGA	40.0	38.1	52.6	57.1	493-537 bp	1.0	49.1
rps15-ycf1_p4	GACCCTCTATTTTCTTCGTTTTCT	TCCGCGATTGAAATTTTGTGAA	37.5	36.4	57.0	57.8	493-537 bp	1.0	49.1
rps15-ycf1_p5	GACCCTCTATTTTCTTCGTTTTC	TCCGCGATTGAAATTTTGTGA	39.1	38.1	55.6	57.1	493-537 bp	1.0	49.1