Hotspot

ccsA-ndhD

SSC · 113120-113348 · 229 bp

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Pi 0.0368

MarkerSeek score 72.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 229
variable_sites: 27
pi: 0.0368
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTGGAGTTGGGACCCAAAAG	GGTTGATAATAGTGGGCGCA	50.0	50.0	57.9	58.0	1111-1138 bp	1.0	84.0
ccsA-ndhD_p2	TTGGAGTTGGGACCCAAAAG	TGGTTGATAATAGTGGGCGC	50.0	50.0	57.9	58.0	1112-1139 bp	1.0	84.0
ccsA-ndhD_p3	CTAACGAAGCATGGGGATCC	GGTTGATAATAGTGGGCGCA	55.0	50.0	58.1	58.0	1133-1160 bp	1.0	83.7
ccsA-ndhD_p4	CTAACGAAGCATGGGGATCC	TGGTTGATAATAGTGGGCGC	55.0	50.0	58.1	58.0	1134-1161 bp	1.0	83.7
ccsA-ndhD_p5	ATTAGTACCCGCCCTTCAGT	GGTTGATAATAGTGGGCGCA	50.0	50.0	58.1	58.0	1498-1525 bp	1.0	83.2