Hotspot

rpl32-trnL(UAG)

SSC · 111253-111954 · 702 bp

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Pi 0.0283

MarkerSeek score 72.80

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 702
variable_sites: 56
pi: 0.0283
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	TCGGAAAAAGAAGAAGCCCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1317-1517 bp	1.0	87.6
rpl32-trnL(UAG)_p2	TCGGAAAAAGAAGAAGCCCC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.1	57.8	1310-1510 bp	1.0	87.1
rpl32-trnL(UAG)_p3	TCGGAAAAAGAAGAAGCCCC	ACTGAAGGGCGGGTACTAAT	50.0	50.0	58.1	58.1	1858-2063 bp	1.0	86.6
rpl32-trnL(UAG)_p4	TCGGAAAAAGAAGAAGCCCC	GATGATATGCCATGCCGCTA	50.0	50.0	58.1	58.0	1370-1556 bp	0.875	82.8
rpl32-trnL(UAG)_p5	CTGGAAGTGGAACGAAAGGT	GATGATATGCCATGCCGCTA	50.0	50.0	57.7	58.0	1331-1517 bp	0.875	81.6