Hotspot

ndhF-rpl32

SSC · 110605-111066 · 462 bp

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Pi 0.026

MarkerSeek score 69.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 462
variable_sites: 34
pi: 0.026
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	ACCGGCCAGTCTTACTATGA	50.0	50.0	58.1	57.8	1111-1311 bp	1.0	87.3
ndhF-rpl32_p2	TCGGAAAAAGAAGAAGCCCC	ACCGGCCAGTCTTACTATGA	50.0	50.0	58.1	57.8	1080-1280 bp	1.0	87.2
ndhF-rpl32_p3	AAAAGCCCACATACGACGAA	ACCGGCCAGTCTTACTATGA	45.0	50.0	57.8	57.8	1115-1315 bp	1.0	86.4
ndhF-rpl32_p4	ACACATCCCAACCAATTCCC	ACCGGCCAGTCTTACTATGA	50.0	50.0	58.1	57.8	1460-1660 bp	0.875	81.2
ndhF-rpl32_p5	CACATCCCAACCAATTCCCA	ACCGGCCAGTCTTACTATGA	50.0	50.0	58.1	57.8	1459-1659 bp	0.875	81.2