Hotspot

trnG(GCC)-trnfM(CAU)

LSC · 35602-35760 · 159 bp

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Pi 0.031

MarkerSeek score 67.70

Primer available yes

Label trnG(GCC)-trnfM(CAU)

Feature Metadata

label_name: trnG(GCC)-trnfM(CAU)
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 17
pi: 0.031
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(GCC)-trnfM(CAU)_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	225-242 bp	1.0	85.4
trnG(GCC)-trnfM(CAU)_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	226-243 bp	1.0	85.4
trnG(GCC)-trnfM(CAU)_p3	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	261-278 bp	1.0	84.7
trnG(GCC)-trnfM(CAU)_p4	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	262-279 bp	1.0	84.7
trnG(GCC)-trnfM(CAU)_p5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	302-319 bp	1.0	83.7