Hotspot

trnT(GGU)-psbD

LSC · 30610-31968 · 1359 bp

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Pi 0.0148
MarkerSeek score 64.70
Primer available yes
Label trnT(GGU)-psbD

Feature Metadata

label_name
trnT(GGU)-psbD
feature_type
igs
region
LSC
length_bp
1359
variable_sites
58
pi
0.0148
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(GGU)-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1511-1535 bp 1.0 83.1
trnT(GGU)-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1514-1538 bp 1.0 82.9
trnT(GGU)-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1501-1525 bp 1.0 82.0
trnT(GGU)-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1504-1528 bp 1.0 81.8
trnT(GGU)-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1488-1512 bp 1.0 81.4