Hotspot

trnH(GUG)-psbA

LSC · 81-402 · 322 bp

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Pi 0.0372
MarkerSeek score 70.50
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
322
variable_sites
27
pi
0.0372
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.93
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 ATCCACTTGGCTACATCCG CTATCGAAGCTCCAACAAATG 52.6 42.9 56.9 55.0 349-376 bp 1.0 50.0
trnH(GUG)-psbA_p2 ATCCACTTGGCTACATCCGC CTATCGAAGCTCCAACAAATG 55.0 42.9 60.2 55.0 349-376 bp 1.0 50.0
trnH(GUG)-psbA_p3 CCACTTGGCTACATCCGC CTATCGAAGCTCCAACAAATG 61.1 42.9 58.2 55.0 347-374 bp 1.0 50.0
trnH(GUG)-psbA_p4 ATCCACTTGGCTACATCCG TATCGAAGCTCCAACAAATG 52.6 40.0 56.9 53.7 348-375 bp 1.0 50.0
trnH(GUG)-psbA_p5 ATCCACTTGGCTACATCCGC TATCGAAGCTCCAACAAATG 55.0 40.0 60.2 53.7 348-375 bp 1.0 50.0