Hotspot

ycf1

SSC · 122318-127777 · 5460 bp

Back to Climacoptera

Pi 0.0197

MarkerSeek score 50.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 107
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGATTTTGTCCGGATCCTCC	ACGAAAGTGAAGAAGAAGAAGA	55.0	36.4	58.4	55.4	150-189 bp	1.0	45.7
ycf1_p2	CGATTTTGTCCGGATCCTCC	ACGAAAGTGAAGAAGAAGAAGAAG	55.0	37.5	58.4	57.2	150-189 bp	1.0	45.7
ycf1_p3	CGATTTTGTCCGGATCCTCC	ACGAAAGTGAAGAAGAAGAAGAA	55.0	34.8	58.4	56.1	150-189 bp	1.0	45.7
ycf1_p4	TCGATTTTGTCCGGATCCTC	ACGAAAGTGAAGAAGAAGAAGA	50.0	36.4	57.4	55.4	151-190 bp	1.0	45.6
ycf1_p5	TCGATTTTGTCCGGATCCTC	ACGAAAGTGAAGAAGAAGAAGAAG	50.0	37.5	57.4	57.2	151-190 bp	1.0	45.6