Hotspot

rpl2

LSC · 83373-84197 · 825 bp

Back to Climacoptera

Pi 0.00848
MarkerSeek score 62.70
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
LSC
length_bp
825
variable_sites
7
pi
0.00848
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 ACGTGTCACAGTTAATTACTCCT TGGGACCTATTATGGGACGT 39.1 50.0 57.5 57.5 1015-1016 bp 1.0 49.1
rpl2_p2 ACGTGTCACAGTTAATTACTCC TGGGACCTATTATGGGACGT 40.9 50.0 56.1 57.5 1015-1016 bp 1.0 45.9
rpl2_p3 ACGTGTCACAGTTAATTACTCCT ATGGGACCTATTATGGGACGT 39.1 47.6 57.5 58.0 1016-1017 bp 1.0 44.8
rpl2_p4 CGTGTCACAGTTAATTACTCCT TGGGACCTATTATGGGACGT 40.9 50.0 55.9 57.5 1014-1015 bp 1.0 43.7
rpl2_p5 AACGTGTCACAGTTAATTACTCCT TGGGACCTATTATGGGACGT 37.5 50.0 58.1 57.5 1016-1017 bp 1.0 43.6