Hotspot

rpl32-trnL-UAG

SSC · 111820-112609 · 790 bp

Back to Climacoptera

Pi 0.0358

MarkerSeek score 70.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 790
variable_sites: 26
pi: 0.0358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.1	881-925 bp	1.0	85.6
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCGTTAA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.0	58.1	883-927 bp	1.0	85.6
rpl32-trnL-UAG_p3	GGGATATTGGGCAGCGTTAA	GTTAAGAGGCCTGACGTAGC	50.0	55.0	58.0	58.1	1417-1457 bp	1.0	84.6
rpl32-trnL-UAG_p4	GGGATATTGGGCAGCGTTAA	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1521-1561 bp	1.0	84.5
rpl32-trnL-UAG_p5	GGGATATTGGGCAGCGTTAA	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1520-1560 bp	1.0	84.5