Hotspot

ndhF-rpl32

SSC · 110563-111666 · 1104 bp

Back to Climacoptera

Pi 0.0296

MarkerSeek score 69.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1104
variable_sites: 30
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGAAGTGGAAGGAAGGGTA	CGCTTTTTGTAAATCGAAGTACG	50.0	39.1	57.6	57.1	1129-1196 bp	1.0	51.7
ndhF-rpl32_p2	TGGAAGTGGAAGGAAGGGTA	ACGAATACGCTTTTTGTAAATCGA	50.0	33.3	57.6	57.8	1136-1203 bp	1.0	49.4
ndhF-rpl32_p3	TGGAAGTGGAAGGAAGGGTA	ACGCTTTTTGTAAATCGAAGT	50.0	33.3	57.6	54.7	1130-1197 bp	1.0	48.5
ndhF-rpl32_p4	CTGGAAGTGGAAGGAAGGGT	CGCTTTTTGTAAATCGAAGTACG	55.0	39.1	58.9	57.1	1130-1197 bp	1.0	47.6
ndhF-rpl32_p5	GGAAGGGTATGATCCATGCA	CGCTTTTTGTAAATCGAAGTACG	50.0	39.1	57.0	57.1	1118-1185 bp	1.0	47.5