Hotspot

trnN-GUU-ndhF

IRb · 106275-108330 · 2056 bp

Back to Climacoptera

Pi 0.0322

MarkerSeek score 70.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2056
variable_sites: 62
pi: 0.0322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TGGGTCTTTTGTCGAAGTGG	TGGCTTTGGTGTTACAAGCT	50.0	45.0	58.0	57.9	2445-2543 bp	1.0	85.4
trnN-GUU-ndhF_p2	GGCAGAATAGCAGAGCAAGT	TGGCTTTGGTGTTACAAGCT	50.0	45.0	58.0	57.9	2339-2433 bp	1.0	85.3
trnN-GUU-ndhF_p3	GCTTCCCTACTCTTAACGGC	TGGCTTTGGTGTTACAAGCT	55.0	45.0	58.1	57.9	2236-2330 bp	1.0	85.2
trnN-GUU-ndhF_p4	GAAGGACCTTTCCCCTTTGG	TGGCTTTGGTGTTACAAGCT	55.0	45.0	58.1	57.9	2523-2621 bp	1.0	85.0
trnN-GUU-ndhF_p5	GAACTTGGGTGTGGGTCTTT	TGGCTTTGGTGTTACAAGCT	50.0	45.0	57.9	57.9	2554 bp	0.5	58.9