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psbT-psbN

LSC · 73357-73421 · 65 bp

Back to Climacoptera

Pi 0.0308

MarkerSeek score 68.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GGAACCCCCGAAAATTCAAAC	TTCGAGGAACACGGAGACTA	47.6	50.0	58.0	57.8	123 bp	1.0	71.5
psbT-psbN_p2	GGAACCCCCGAAAATTCAAAC	TCGAGGAACACGGAGACTAG	47.6	55.0	58.0	58.3	122 bp	1.0	70.9
psbT-psbN_p3	GGAACCCCCGAAAATTCAAAC	AGAGATCCCTTCGAGGAACA	47.6	50.0	58.0	57.5	132 bp	1.0	68.7
psbT-psbN_p4	GGAACCCCCGAAAATTCAAAC	GGCAACCCTCTCAACAACTA	47.6	50.0	58.0	57.4	152 bp	1.0	68.3
psbT-psbN_p5	GGAACCCCCGAAAATTCAAAC	GAGATCCCTTCGAGGAACAC	47.6	55.0	58.0	57.4	131 bp	1.0	68.2