Hotspot

trnS-GGA-rps4

LSC · 44530-44873 · 344 bp

Back to Climacoptera

Pi 0.0293

MarkerSeek score 63.10

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 10
pi: 0.0293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	TGACTCTTCACCCATTCCCA	50.0	50.0	58.1	58.3	497-500 bp	1.0	79.3
trnS-GGA-rps4_p2	GGCTTTTGTTTACCGAGGGT	TGACTCTTCACCCATTCCCA	50.0	50.0	58.4	58.3	496-499 bp	1.0	76.9
trnS-GGA-rps4_p3	AGGCTTTTGTTTACCGAGGG	TTGACTCTTCACCCATTCCC	50.0	50.0	58.1	57.1	498-501 bp	1.0	74.3
trnS-GGA-rps4_p4	AGGCTTTTGTTTACCGAGGG	GACTCTTCACCCATTCCCAT	50.0	50.0	58.1	56.9	496-499 bp	1.0	72.5
trnS-GGA-rps4_p5	TTCGAATCCCTCTCTTTCCG	TGACTCTTCACCCATTCCCA	50.0	50.0	57.0	58.3	477-480 bp	1.0	72.4