Hotspot

ycf3-trnS-GGA

LSC · 43876-44441 · 566 bp

Back to Climacoptera

Pi 0.0236

MarkerSeek score 73.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 12
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	682-739 bp	1.0	77.7
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	681-738 bp	1.0	77.6
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	703-760 bp	1.0	77.2
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	702-759 bp	1.0	77.2
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTCAA	50.0	45.0	57.1	57.8	653-710 bp	1.0	76.9