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rps14-psaB

LSC · 36439-36559 · 121 bp

Back to Climacoptera

Pi 0.0413

MarkerSeek score 76.30

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 5
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TCTCTTCTTCTCCCGCTGAA	ACCAGTGGCTCTTTCCATTG	50.0	50.0	58.1	58.1	278 bp	1.0	80.2
rps14-psaB_p2	TCTCTTCTTCTCCCGCTGAA	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.1	58.1	277 bp	1.0	80.2
rps14-psaB_p3	TCTCTTCTTCTCCCGCTGAA	TGGATTAGCCCACTTTTCCG	50.0	50.0	58.1	57.9	242 bp	1.0	80.1
rps14-psaB_p4	TCTCTTCTTCTCCCGCTGAA	GGATTAGCCCACTTTTCCGT	50.0	50.0	58.1	57.9	241 bp	1.0	80.1
rps14-psaB_p5	GCTCAACGATGCTACTTTGC	CCAGTGGCTCTTTCCATTGT	50.0	50.0	57.8	58.1	352 bp	1.0	79.1