Hotspot

rpoB-trnC-GCA

LSC · 26419-27564 · 1146 bp

Back to Climacoptera

Pi 0.0229

MarkerSeek score 58.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 26
pi: 0.0229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	AGGCCCAGTTAGTAACCACT	50.0	50.0	58.0	58.0	1969-1976 bp	1.0	81.1
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	GGAATGGAGAGGTCTCGGTA	50.0	55.0	58.0	57.9	1943-1950 bp	1.0	80.9
rpoB-trnC-GCA_p3	CCCGGGCTTTGCAATATTTG	AGGCCCAGTTAGTAACCACT	50.0	50.0	58.1	58.0	1849-1856 bp	1.0	80.9
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1720-1722 bp	1.0	80.9
rpoB-trnC-GCA_p5	CCCGGGCTTTGCAATATTTG	GGAATGGAGAGGTCTCGGTA	50.0	55.0	58.1	57.9	1823-1830 bp	1.0	80.7