Hotspot

trnS-GCU-trnG-GCC

LSC · 7900-8741 · 842 bp

Back to Climacoptera

Pi 0.0351

MarkerSeek score 70.40

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 29
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	965-967 bp	1.0	54.2
trnS-GCU-trnG-GCC_p2	CGAATGACTCGTACAACGGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	935-937 bp	1.0	53.5
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	968-970 bp	1.0	53.2
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	967-969 bp	1.0	53.2
trnS-GCU-trnG-GCC_p5	TCGAAACGGAAAGAGAGGGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.4	55.5	970-972 bp	1.0	52.4