Hotspot

trnK-UUU-rps16

LSC · 4146-4818 · 673 bp

Back to Climacoptera

Pi 0.0244
MarkerSeek score 72.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
673
variable_sites
16
pi
0.0244
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TGCTCAACCTACCGAAACTG 50.0 50.0 58.2 57.8 765-780 bp 1.0 80.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TGCTCAACCTACCGAAACTG 50.0 50.0 58.2 57.8 764-779 bp 1.0 80.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCTCAACCTACCGAAACTGT 50.0 50.0 58.2 57.8 764-779 bp 1.0 80.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCTCAACCTACCGAAACTGT 50.0 50.0 58.2 57.8 763-778 bp 1.0 80.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GTGCTCAACCTACCGAAACT 50.0 50.0 58.2 57.8 765-780 bp 1.0 80.9