Hotspot

rps3

LSC · 85645-86325 · 681 bp

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Pi 0.00441

MarkerSeek score 53.80

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 681
variable_sites: 3
pi: 0.00441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGTTCCGTTACTGGGGTAGA	ATATAGACCCTCTCGCGTGG	50.0	55.0	57.7	58.5	815 bp	1.0	74.1
rps3_p2	TGTTCCGTTACTGGGGTAGA	CCCTCTCGCGTGGTTAAAAA	50.0	50.0	57.7	58.5	808 bp	1.0	74.0
rps3_p3	TTTTGTTCCGTTACTGGGGT	ATATAGACCCTCTCGCGTGG	45.0	55.0	57.3	58.5	818 bp	1.0	70.7
rps3_p4	TTTTGTTCCGTTACTGGGGT	CCCTCTCGCGTGGTTAAAAA	45.0	50.0	57.3	58.5	811 bp	1.0	70.6
rps3_p5	TGTTCCGTTACTGGGGTAGA	TATAGACCCTCTCGCGTGG	50.0	57.9	57.7	58.0	814 bp	1.0	69.7