Hotspot

rps15

SSC · 126270-126542 · 273 bp

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Pi 0.00733

MarkerSeek score 44.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 2
pi: 0.00733
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	CACCAATAATGCCTACCCCC	ACATGTACCCACGATTGCAA	55.0	45.0	58.0	57.8	1398-1402 bp	1.0	78.8
rps15_p2	CCACCAATAATGCCTACCCC	ACATGTACCCACGATTGCAA	55.0	45.0	58.0	57.8	1399-1403 bp	1.0	78.8
rps15_p3	ACCCCCTCAACTCGTTCTAA	ACATGTACCCACGATTGCAA	50.0	45.0	58.0	57.8	1384-1388 bp	1.0	78.7
rps15_p4	GCTATCCGGCTCAATTCCAA	ACATGTACCCACGATTGCAA	50.0	45.0	58.0	57.8	1102-1106 bp	1.0	78.6
rps15_p5	TCTGTGCACCAATATCTGCC	ACATGTACCCACGATTGCAA	50.0	45.0	58.0	57.8	1152-1156 bp	1.0	78.5