Hotspot

clpP

LSC · 72689-74720 · 2032 bp

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Pi 0.00246

MarkerSeek score 59.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2032
variable_sites: 5
pi: 0.00246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGGCTGGTTTAGATCGATC	ACGTTTCCACATCAAAGTGA	50.0	40.0	57.1	55.5	2184-2186 bp	1.0	53.1
clpP_p2	TGGGCTGGTTTAGATCGATC	ACGTTTCCACATCAAAGTGAA	50.0	38.1	57.1	56.3	2184-2186 bp	1.0	51.4
clpP_p3	GGGCTGGTTTAGATCGATCT	ACGTTTCCACATCAAAGTGA	50.0	40.0	56.8	55.5	2183-2185 bp	1.0	50.7
clpP_p4	AATGGGCTGGTTTAGATCGA	ACGTTTCCACATCAAAGTGA	45.0	40.0	56.6	55.5	2186-2188 bp	1.0	49.2
clpP_p5	TGGGCTGGTTTAGATCGATC	ACGTTTCCACATCAAAGTGAAA	50.0	36.4	57.1	57.0	2184-2186 bp	1.0	49.2