Hotspot

ndhF-rpl32

SSC · 115330-116261 · 932 bp

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Pi 0.00429

MarkerSeek score 61.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 932
variable_sites: 4
pi: 0.00429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACATACGGCGAAGGTTTT	AGCTTTTAAGGCTGCCCAAT	50.0	45.0	58.8	58.1	1145-1154 bp	1.0	73.1
ndhF-rpl32_p2	CACATACGGCGAAGGTTTTT	AGCTTTTAAGGCTGCCCAAT	45.0	45.0	57.0	58.1	1144-1153 bp	1.0	72.0
ndhF-rpl32_p3	CCACATACGGCGAAGGTTTT	AAAGCTTTTAAGGCTGCCCA	50.0	45.0	58.8	58.3	1147-1156 bp	1.0	71.3
ndhF-rpl32_p4	CCACATACGGCGAAGGTTTT	AAGCTTTTAAGGCTGCCCAA	50.0	45.0	58.8	58.3	1146-1155 bp	1.0	71.3
ndhF-rpl32_p5	ACAAAAGGTATGATCCACGCA	AGCTTTTAAGGCTGCCCAAT	42.9	45.0	57.9	58.1	1066-1075 bp	1.0	71.3