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petA-psbJ

LSC · 65465-66439 · 975 bp

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Pi 0.00308

MarkerSeek score 60.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 3
pi: 0.00308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1085-1094 bp	1.0	75.5
petA-psbJ_p2	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1086-1095 bp	1.0	73.2
petA-psbJ_p3	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1113-1122 bp	1.0	73.1
petA-psbJ_p4	ACATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	1118-1127 bp	1.0	72.8
petA-psbJ_p5	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	1112-1121 bp	1.0	72.7