Hotspot

atpB-rbcL

LSC · 56848-57629 · 782 bp

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Pi 0.00256

MarkerSeek score 60.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 2
pi: 0.00256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATTTGAACGATACACCCCGG	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	906-908 bp	1.0	72.0
atpB-rbcL_p2	GTCCCAGGACCAGAAGTAGT	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	864-866 bp	1.0	71.8
atpB-rbcL_p3	AGTACAGAAGTCCCAGGACC	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	873-875 bp	1.0	71.7
atpB-rbcL_p4	TACAGAAGTCCCAGGACCAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	871-873 bp	1.0	71.7
atpB-rbcL_p5	ATTTGAACGATACACCCCGG	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	905-907 bp	1.0	71.7