Hotspot

ndhC-trnV-UAC

LSC · 52626-53828 · 1203 bp

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Pi 0.00592
MarkerSeek score 63.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1203
variable_sites
6
pi
0.00592
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCTACTCGGGGCTAAAACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.1 57.8 1179-1341 bp 1.0 82.3
ndhC-trnV-UAC_p2 TTGCTACTCGGGGCTAAAAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1181-1343 bp 1.0 82.0
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1172-1334 bp 1.0 81.7
ndhC-trnV-UAC_p4 CTCTGGTCCTTTGCTACTCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.7 57.8 1191-1353 bp 1.0 80.5
ndhC-trnV-UAC_p5 CTTTGCTACTCGGGGCTAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1183-1345 bp 1.0 79.6