Hotspot

psaA-ycf3

LSC · 43711-44458 · 748 bp

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Pi 0.00535

MarkerSeek score 61.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 4
pi: 0.00535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	931-936 bp	1.0	79.4
psaA-ycf3_p2	TTACGGGATCCCTGTCTACC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	57.9	58.0	904-909 bp	1.0	79.4
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	913-918 bp	1.0	79.4
psaA-ycf3_p4	TTACGGGATCCCTGTCTACC	GGAAACAGGCTATAGCGCTT	55.0	50.0	57.9	58.0	886-891 bp	1.0	79.4
psaA-ycf3_p5	GTCTGGCCCATTCCTCAAAA	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	933-938 bp	1.0	78.6