Hotspot

atpH-atpI

LSC · 14385-15406 · 1022 bp

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Pi 0.00489

MarkerSeek score 61.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1022
variable_sites: 5
pi: 0.00489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1156-1181 bp	1.0	79.0
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	1212-1237 bp	1.0	78.4
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1157-1182 bp	1.0	77.8
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CCCGTCATGTTCCTTGGATT	55.0	50.0	58.2	57.9	1216-1241 bp	1.0	77.8
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.8	57.9	1171-1196 bp	1.0	77.6