Hotspot

trnK-UUU-rps16

LSC · 4360-5150 · 791 bp

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Pi 0.00379
MarkerSeek score 60.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
791
variable_sites
3
pi
0.00379
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AAGGGCGGAGGTTTTTAAGT 50.0 45.0 58.0 57.3 895 bp 1.0 74.6
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC AAGGGCGGAGGTTTTTAAGT 50.0 45.0 58.0 57.3 896 bp 1.0 74.6
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC AAGGGCGGAGGTTTTTAAGT 50.0 45.0 58.0 57.3 900 bp 1.0 74.6
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT AAGGGCGGAGGTTTTTAAGT 50.0 45.0 58.0 57.3 898 bp 1.0 74.6
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC AAGGGCGGAGGTTTTTAAGT 55.0 45.0 57.9 57.3 907 bp 1.0 74.3