Hotspot

trnH-GUG-psbA

LSC · 76-480 · 405 bp

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Pi 0.0123

MarkerSeek score 61.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 5
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	482-483 bp	1.0	79.2
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	483-484 bp	1.0	78.9
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	475-476 bp	1.0	77.6
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	531-532 bp	1.0	77.0
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	532-533 bp	1.0	76.8