Hotspot

ycf1

SSC · 121923-127493 · 5571 bp

Back to Clerodendrum

Pi 0.0174

MarkerSeek score 55.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 224
pi: 0.0174
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTATTCTCTTGCGTGGTGA	ATATATGGCAAACCCGACCG	50.0	50.0	58.0	57.8	957 bp	0.8	73.7
ycf1_p2	AGCTATTCTCTTGCGTGGTG	ATATATGGCAAACCCGACCG	50.0	50.0	58.0	57.8	958 bp	0.8	73.6
ycf1_p3	CAGCTATTCTCTTGCGTGGT	ATATATGGCAAACCCGACCG	50.0	50.0	58.0	57.8	959 bp	0.8	73.6
ycf1_p4	GCTATTCTCTTGCGTGGTGA	GCAAACCCGACCGATAGAAA	50.0	50.0	58.0	58.3	950 bp	0.8	73.0
ycf1_p5	AGCTATTCTCTTGCGTGGTG	GCAAACCCGACCGATAGAAA	50.0	50.0	58.0	58.3	951 bp	0.8	72.9