Hotspot

ndhF

SSC · 109061-111286 · 2226 bp

Back to Clerodendrum

Pi 0.0111

MarkerSeek score 50.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 58
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATACCGTCGCCCCTTTTTAC	AACTTGTCAAACTCGGCACA	50.0	45.0	58.0	58.3	2917-2955 bp	1.0	80.8
ndhF_p2	ATACCGTCGCCCCTTTTTAC	ACTTGTCAAACTCGGCACAA	50.0	45.0	58.0	58.3	2916-2954 bp	1.0	80.8
ndhF_p3	ACAGGAACAAGAGGGATCCA	AACTTGTCAAACTCGGCACA	50.0	45.0	58.0	58.3	2804-2842 bp	1.0	80.8
ndhF_p4	ACAGGAACAAGAGGGATCCA	ACTTGTCAAACTCGGCACAA	50.0	45.0	58.0	58.3	2803-2841 bp	1.0	80.8
ndhF_p5	AAGAGGGATCCACCGAAGAA	ACTTGTCAAACTCGGCACAA	50.0	45.0	58.0	58.3	2797-2833 bp	0.8	72.3