Hotspot

trnL-UAG-ccsA

SSC · 112554-112658 · 105 bp

Back to Clerodendrum

Pi 0.0225

MarkerSeek score 66.60

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 105
variable_sites: 4
pi: 0.0225
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGAAAGGGTCGAAAAAGAAA	50.0	35.0	57.9	53.2	211-223 bp	1.0	46.4
trnL-UAG-ccsA_p2	TAGACACGCTGCTCTTAGGA	TTGAAAGGGTCGAAAAAGAA	50.0	35.0	57.9	53.2	212-224 bp	1.0	46.4
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	TGAAAGGGTCGAAAAAGAAA	45.0	35.0	57.8	53.2	218-230 bp	1.0	45.8
trnL-UAG-ccsA_p4	AAATTGGTAGACACGCTGCT	TTGAAAGGGTCGAAAAAGAA	45.0	35.0	57.8	53.2	219-231 bp	1.0	45.8
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	TGAAAGGGTCGAAAAAGAAA	55.0	35.0	57.8	53.2	212-224 bp	1.0	45.8