Hotspot

rps19-rpl2

IRb · 83435-83494 · 60 bp

Back to Clerodendrum

Pi 0.0302

MarkerSeek score 62.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 60
variable_sites: 3
pi: 0.0302
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGATTGGCTACAAAAGGATT	GATTGTTCGTCGCCGTAGTA	35.0	50.0	52.7	57.8	119-126 bp	1.0	46.0
rps19-rpl2_p2	TGATTGGCTACAAAAGGATT	CACAACCCCTTGGGGTTATC	35.0	55.0	52.7	58.2	188-195 bp	1.0	44.2
rps19-rpl2_p3	TGATTGGCTACAAAAGGATTT	CACAACCCCTTGGGGTTATC	33.3	55.0	53.6	58.2	188-195 bp	1.0	44.2
rps19-rpl2_p4	TGATTGGCTACAAAAGGATT	CGAGCCCCAATTGGTAGAAA	35.0	50.0	52.7	57.9	214-221 bp	1.0	43.7
rps19-rpl2_p5	TGATTGGCTACAAAAGGATTT	CGAGCCCCAATTGGTAGAAA	33.3	50.0	53.6	57.9	214-221 bp	1.0	43.7