Hotspot

trnT-UGU-trnL-UAA

LSC · 46389-47072 · 684 bp

Back to Clerodendrum

Pi 0.0173

MarkerSeek score 66.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 29
pi: 0.0173
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	733-772 bp	1.0	82.7
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	TAGTTCCGGGGTTTCTCTGA	55.0	50.0	57.9	57.7	807-846 bp	1.0	81.7
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	765-804 bp	1.0	81.6
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	727-766 bp	1.0	81.5
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	TAGTTCCGGGGTTTCTCTGA	50.0	50.0	57.8	57.7	839-878 bp	1.0	80.6